Record Display for the EPA National Library Catalog

RECORD NUMBER: 1563 OF 1580

OLS Field Name OLS Field Data
Main Title Using Neuroblastoma Cell Lines to Examine Organophosphate Neurotoxicity.
Author Veronesi, B. ; Ehrich, M. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Virginia-Maryland Regional Coll. of Veterinary Medicine, Blacksburg, VA.
Publisher c1993
Year Published 1993
Report Number EPA/600/J-93/332;
Stock Number PB93-229508
Additional Subjects Toxicity ; Nervous system ; Neuroblastoma ; Organophosphate insecticides ; Cultured tumor cells ; In vitro analysis ; Esterases ; Cholinesterase inhibitors ; Reprints ;
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB93-229508 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 11/22/1993
Collation 10p
Abstract
The paper describes the initial characterization of neuroblastoma cell lines to address several aspects of organophosphate neurotoxicity. Several commercially available human and mouse cell lines (i.e., SY5Y, IMR-32, SK-N-MC, NB41A3) were evaluated for their target esterase activities (i.e., cholinesterase, neurotoxic esterase, carboxylesterase), and of these cells, a human (SY5Y) and mouse (NB41A3) neuroblastoma cell line clone were used to establish an IC50 cytoxicity profile for a variety of organophosphates insecticides (e.g., parathion, paraoxon, diisopropylphosphorofluoridate and mipafox). The human neuroblastoma cell line clone (SY5Y) was further used to distinguish between neuropathy-causing OPs and cholinesterase inhibitors. These initial data support the use of neuroblastoma cell lines as effective test models for organophosphate neurotoxicity.