A demonstration of screening technologies for determining the presence of dioxin and dioxin-like compounds in soil and sediment was conducted under the U.S. Environmental Protection Agencys (EPAs) Superfund Innovative Technology Evaluation Program in Saginaw, Michigan in 2004. The objectives of the demonstration included evaluating each participating technologys accuracy, precision, sensitivity, sample throughput, tendency for matrix effects, and cost. The test also included an assessment of how well the technologys results compared to those generated by established laboratory methods using high-resolution mass spectrometry (HRMS). The demonstration objectives were accomplished by evaluating the results generated by each technology from 209 soil, sediment, and extract samples. The test samples included performance evaluation (PE) samples (i.e., contaminant concentrations were certified or the samples were spiked with known contaminants) and environmental samples collected from 10 different sampling locations. The PE and environmental samples were distributed to the technology developers in blind, random order. One of the participants in the original SITE demonstration was Hybrizyme Corporation, which demonstrated the use of the AhRC PCR Kit. The AhRC PCR Kit was a technology that reported the concentration of aryl hydrocarbon receptor (AhR) binding compounds in a sample, with units reported as Ah Receptor Binding Units (AhRBU). At the time of the original demonstration, this particular technology was intended for use as a screening tool to rank samples from those inducing the greatest AhR activity to those inducing the least AhR activity rather than to provide highly quantitative dioxin concentration in units of toxicity equivalents (TEQ). After the SITE Dioxin demonstration, this technology was exclusively licensed to Eichrom Technologies. Eichrom focused their efforts on developing optimal sample preparation procedures for the assay and reporting TEQ values
instead of AhRBU. The technology is now marketed under the trade name Procept Rapid Dioxin Assay. This report describes the experimental design of the site-specific study, the analytical methods used, and comparisons of the TEQD/F results from the HRMS data to those reported by Eichrom Technologies Procept Rapid Dioxin Assay. The data generated and evaluated during the site-specific study showed that the TEQ data produced by the Procept Rapid Dioxin Assay was more comparable to the HRMS TEQD/F data than was the data reported using the Hybrizyme AhRC PCR Kit in the original SITE demonstration. The Procept Rapid Dioxin Assay could be used as an effective screening tool to determine areas of greatest concern for cleanup at a site and could help to minimize the number of more expensive analyses needed for specific analytes, particularly considering that the cost and the time to analyze samples is significantly less than that of HRMS analyses.