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RECORD NUMBER: 26 OF 49

OLS Field Name OLS Field Data
Main Title Direct Analysis of Rat Bile for Acetaminophen and Two of Its Conjugated Metabolites via Thermospray Liquid Chromatography/Mass Spectrometry.
Author Betowski, L. D. ; Korfmacher, W. A. ; Lay, J. O. ; Potter, D. W. ; Hinson, J. A. ;
CORP Author Environmental Monitoring Systems Lab., Las Vegas, NV. ;National Center for Toxicological Research, Jefferson, AR.
Year Published 1987
Report Number EPA/600/J-87/351;
Stock Number PB88-215306
Additional Subjects Bile ; Mass spectroscopy ; Acetanilides ; Chemical analysis ; Metabolism ; Laboratory animals ; Reprints ; Acetaminophen ; Acetanilide/hydroxy ; Acetamide/hydroxyphenyl ; High pressure liquid chromatography ; Thermospray liquid chromatography ; Glutathione-acetaminophen conjugate ; Glucuronide-acetaminophen conjagate
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB88-215306 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 12/29/1988
Collation 7p
Abstract
Bile from rats treated with acetaminophen was analyzed by direct injection onto a thermospray liquid chromatography/mass spectrometry system. Two conjugated metabolites of acetaminophen were separated by the high-pressure liquid chromatographic system and analyzed by mass spectrometry. The conjugates were identified as the glutathione-acetaminophen conjugate and the glucuronide-acetaminophen conjugate by comparison of the chromatographic retention times and the mass spectra to that of the synthetic standards. No evidence of acetaminophen metabolites was observed when bile samples were subjected to direct analysis by fast atom bombardment mass spectrometry.