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RECORD NUMBER: 14 OF 72

OLS Field Name OLS Field Data
Main Title Chronic Toxicity Studies on 1,3,5-Trinitrobenzene in Fischer 344 Rats.
Author Reddy, T. V. ; Daniel, F. B. ; Olson, G. R. ; Wiechman, B. ; Torsella, J. ;
CORP Author Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Publisher Feb 96
Year Published 1996
Report Number MIPR-93MM3558;
Stock Number AD-A315 216/2
Additional Subjects Benzene ; Toxicity ; Methemoglobin ; Brain ; Rats ; Powders ; Abstracts ; Chemistry ; Deposition ; Drops ; Weight ; Adverse conditions ; Laboratories ; Males ; Immunology ; Pigments ; Diet ; Body weight ; Females ; Sex ; Spleen ; Histology ; Kidneys ; Hematology ; Trinitrobenzene ; Chronic oral toxicity ; Alpha 2u globulin ; Hyaline droplets ; Tnb(Trinitrobenzene)
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NTIS  AD-A315 216/2 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 02/22/1997
Collation 806p
Abstract
Chronic toxic effects of I ,3,5-tnnitriotrobenzene (TNB) in male and female Fischer rats were evaluated by feeding certified powdered laboratory chow diet supplemented with varied concentrations of TNB (0, 5, 60 and 300 mg/kg diet). The study was designed to accommodate three interim sacrifices (10 rats/group/sex) at 90, 180 and 365 days. The final sacrifice was performed after two years. All data related to these interim sacrifices are presented independently in appendices J to L. The calculated average TNB consumption for females was 0.23, 2.68 and 13.31 mg/kg/day and was 0.22, 2.64 and 13.44 mg/kg/day for males. Terminal body weights were significantly decreased in both sexes in the 300 mg/kg group. Relative spleen weights were decreased in both sexes in the 300 mg/kg group while brain weights were increased in females in this same group. Methemoglobin was increased in both sexes in the 300 mg/kg group while other hematological effects noted at the interim sacrifice times were not evident at two years. Histopathological examinations suggested treatment related changes in both sexes involving the kidneys (cytoplasmic/hyaline droplets) in the 60 and 300 mg/kg groups and the spleen (erythroid cell hyperplasia and pigment deposition) in the 300 mg/kg group. The cytoplasmic/hyaline droplets were characterized by immunohistochemistry as alpha-2u-globulin. These renal droplets were also noted at the interim sacrifice times. A no observed adverse effect level (NOAEL) was established in this study at 2.68 mg/kg b.w./day for F-344 rats administered TNB for two years.