||Characterization of Sulfhydryl Proteins Involved in the Maintenance of Flagellar Straightness in Hamster Spermatozoa.
Cornwall, G. A. ;
Chang., T. S. K. ;
||Johns Hopkins Hospital, Baltimore, MD.;Health Effects Research Lab., Research Triangle Park, NC.;National Institutes of Health, Bethesda, MD.
||EPA-R-812765 ;PHS-HD-22761; EPA/600/J-90/239;
Molecular weight ;
Sulfhydryl compounds ;
Sperm tail ;
Sperm motility ;
Two-dimensional polyacrylamide gel electrophoresis ;
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Hamster caput epididymal spermatozoa exhibit a marked 90-180 degree bend when induced to acquire progressive motility in vitro (Cornwall et al, 1988). Flagellar bending is prevented by oxidizing sperm sulfhydryl (SH) groups with diamide. In the study, the authors examined the SH proteins involved in sperm flagellar bending using two-dimensional polyacrylamide gel electrophoresis and monobromobimane, an SH-specific fluorescent dye. Proteins extracted from samples containing bent caput spermatozoa contained more reduced SH than the same proteins extracted from straight caput spermatozoa. To further characterize these sperm SH proteins, caudal epididymal spermatozoa that exhibited straight flagella were induced to undergo flagellar bending by treatment with the SH reductant dithiothreitol.