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RECORD NUMBER: 466 OF 852

OLS Field Name OLS Field Data
Main Title Measurement of Methanotroph and Methanogen Signature Phosopholipids for Use in Assessment of Biomass and Community Structure in Model Systems.
Author Nichols, P. D. ; Mancuso, C. A. ; White, D. C. ;
CORP Author Florida State Univ., Tallahassee. Dept. of Biological Science. ;Commonwealth Scientific and Industrial Research Organization, Hobart (Australia). Div. of Oceanography. ;Tennessee Univ., Knoxville. Inst. for Applied Microbiology.;Robert S. Kerr Environmental Research Lab., Ada, OK.
Year Published 1987
Report Number EPA-R-812504; EPA/600/J-87/359;
Stock Number PB88-219811
Additional Subjects Soils ; Biomass ; Spirillacea ; Natural gas ; Biochemistry ; Fatty acids ; Lipids ; Digestion(Biology) ; Methane ; Profiles ; Oxidation reduction reactions ; Graphs(Charts) ; Reprints ; Methanogea
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB88-219811 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 12/29/1988
Collation 14p
Abstract
Methanotrophic biomass and community structure were assessed for a soil column enriched with natural gas. An increase in microbial biomass, based on phospholipid ester-linked fatty acids (PLFA), was apparent for the natural gas-enriched column relative to a control column and untreated surface soil. Following GC-MS analyses of the derivatized monounsaturated fatty acids, the major component (22 percent of the PLFA) of the natural gas-enriched column was identified as 18:1 delta 10C. The relatively novel fatty acid has only been previously reported as a major component in methanotrophs. Its presence in the soil, together with other supportive evidence, implies that the microbial metabolic group makes a large contribution to the column flora. Other microbial groups were also recognized and differences compared between the soils analyzed. (Copyright (c) 1987 Pergamon Journals Ltd.)