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RECORD NUMBER: 5 OF 6

OLS Field Name OLS Field Data
Main Title Interaction of Haloacetonitriles with Glutathione and Glutathione-S-Transferase.
Author Lin, E. L. C. ; Guion, C. W. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1989
Year Published 1989
Report Number EPA/600/J-89/042;
Stock Number PB90-103615
Additional Subjects Glutathione ; Halogen organic compounds ; Water pollution ; Acetonitrile ; Rats ; Metabolism ; Enzyme inhibitors ; Toxicity ; Reprints ; Glutathione transferases ; Liver microsomes ; NADP ; Biotransformation
Holdings
Library Call Number Additional Info Location Last
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Status
NTIS  PB90-103615 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 03/10/1990
Collation 6p
Abstract
The interactions of reduced glutathione (GSH) with haloacetonitriles (HAN) were carried out under various conditions and the depletion of GSH was determined. Haloacetonitriles reacted directly with glutathione in the absence of tissue extracts. In the presence of cytosolic protein, the depletion of GSH by MCAN was increased, but GSH depletion by DBAN and TCAN was not altered. In the presence of microsomes and a NADPH regenerating system, the consumption of GSH by MCAN and DCAN was increased whereas GSH consumption by DBAN and TCAN was decreased, indicating the biotransformation of HAN to metabolites with different reactivities towards GSH. In the presence of bovine serum albumin, the consumption of GSH by TCAN was decreased while no difference was observed in the depletion of GSH by DBAN. All haloacetonitriles inhibited glutathione-S-transferase activities in vitro. TCAN was the most potent inhibitor, followed by DBAN, BCAN, and MCAN, with DCAN was the least reactive.