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RECORD NUMBER: 6 OF 7

OLS Field Name OLS Field Data
Main Title Preparation and Regeneration of Protoplasts of 'Colletotrichum gloeosporioides' f. p. 'Aeschynomene'.
Author TeBeest, D. O. ; Weidemann, G. J. ;
CORP Author Arkansas Univ., Fayetteville. Dept. of Plant Pathology.;Environmental Research Lab., Gulf Breeze, FL.
Publisher c1992
Year Published 1992
Report Number EPA-R-814402; EPA/600/J-92/216;
Stock Number PB92-195734
Additional Subjects Protoplasts ; Plant diseases ; Virulence ; Spores ; Cultured cells ; Beta-glucuronidase ; Chitinase ; Osmolar concentration ; Reprints ; Colletotrichum gloesporioides ; Aeschynomene virginica
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NTIS  PB92-195734 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 08/22/1992
Collation 8p
Abstract
Protoplasts were produced from conidia of Collectotrichum gloespoiodes f. sp. aeschynomenc, a fungal plant pathogen of Aeschynomene virginica, during treatment with Novozym 234 or a mixture of chitinase and beta-glucuronidase after pretreatment with 2-mercaptoethanol. Protoplasts were optimally stabilized in 1.2 M mannitol after release from conidia but regenerated and reverted to hyphae optimally on 0.7 M sucrose. Approximately 84% of the protoplasts regenerated cell walls and reverted to hyphal colonies on 0.7 M sucrose. The osmotic stabilizer and molarity of the stabilizer affected regeneration and reversion to colonies. Microscopic studies of the nuclear content of conidia protoplasts showed that the number of nuclei in protoplasts was similar to the number of nuclei in conidia from which they were produced. Of the 209 colonies grown from reverted protoplasts, all were as pathogenic to A. virginica as the wild-type parent, and all resembled the wild-type strain from which they were produced. The development of an efficient technique to produce protoplasts enables future research on the genetics of the fungus. (Copyright (c) 1990, by The New York Botanical Garden, Bronx, NY.)