||Evaluation of the Dorset Sheep as a Predictive Animal Model for the Response of Glucose-6-Phosphate Dehydrogenase-Deficient Human Erythocytes to a Proposed Systemic Toxic Ozone Intermediate, Methyl Oleate Ozonide.
Calabrese, E. J. ;
Williams, P. S. ;
Moore, G. S. ;
||Massachusetts Univ., Amherst. Dept. of Public Health.;Health Effects Research Lab., Research Triangle Park, NC.
Blood cells ;
Oleic acid/methyl-ozonyl ;
Animal models ;
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Erythrocytes of both glucose-6-phosphate dehydrogenase (G-6-PD)-deficient humans and Dorest sheep, an animal model with an erythrocyte G-6-PD deficiency, responded in a dose-dependent manner to the oxidant stress of methyl oleate ozonide (MOO) as measured by decreases in G-6-PD activity, increases in methemoglobin (METHB) levels, and decreases in GSH. However, the human G-6-PD-deficient erythrocytes were considerably more sensitive to the formation of METHB than the sheep erythrocytes while the reverse was the case for the GSH parameter. The results suggest a qualitative difference in the response of sheep erythrocytes and human G-6-PD-deficient erythrocytes to MOO that seriously questions the value of the sheep erythrocyte as a quantitatively accurate predictive model. (Copyright (c) 1983 by Academic Press, Inc.)