Main Title |
In vitro Competition of Natural 'Autographa Californica' Nuclear Polyhedrosis Virus and a Recombinant Expressing a Polyhedrin-Beta Galactosidase Fusion Protein. |
Author |
Kawanishi, C. Y. ;
Huang, Y. S. ;
Bobseine, K. L. ;
Setzer, W. ;
|
CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. |
Publisher |
Nov 88 |
Year Published |
1988 |
Report Number |
EPA/600/D-88/259; |
Stock Number |
PB89-129084 |
Additional Subjects |
Polyhedrosis viruses ;
Recombinant proteins ;
Genetic intervention ;
Selection(Genetics) ;
Genetic recombination ;
Genetic vectors ;
Autographa californica
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB89-129084 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
9p |
Abstract |
The paper describes results of experiments conducted to investigate the kinetics of in vitro competition between natural progenitor Autographa californica (Acv E-2) and the recombinant Ac360-Bgal virus strains. The following conclusions can be drawn from the analysis: Selection pressure appeared to be fairly constant throughout the experiment; Selection was virtually unidirectional in favor of the natural strain regardless of the starting ratio, and consequently, the natural strain eventually predominated; The observed high frequency of mixed phenotype plaques appear to be influenced by some combinational phenomenon other than recombination at the DNA level; and The frequency of mixed phenotype plaques are also affected by passage. All evidence in the experiment points toward a disfavored recombinant genotype which appears not to be capable of competing effectively in vitro. The data present information that can be utilized as a basis for assessing the potential health effects of baculovirus expression vectors. |