Record Display for the EPA National Library Catalog

RECORD NUMBER: 4 OF 11

OLS Field Name OLS Field Data
Main Title Generation of Recombinant Baculovirus via Liposome-Mediated Transfection.
Author Hartig, P. ; Cardon, M. ; Kawanishi, C. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Developmental Toxicology Div.
Publisher c1991
Year Published 1991
Report Number EPA/600/J-92/025;
Stock Number PB92-143973
Additional Subjects Baculoviridae ; Genetic recombination ; Liposomes ; Transfection ; Genetic vectors ; Viral DNA ; Plasmids ; Recombinant proteins ; Post-translational protein processing ; Calcium phosphates ; Reprints ; Spodoptera frugiperda
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB92-143973 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 08/28/1992
Collation 5p
Abstract
Baculovirus expression vectors have become a popular method of producing recombinant proteins. Production of recombinant virus requires the transfection of both the native viral DNA and a transfer plasmid into insect cells where recombination takes place. While several methods of transfecting insect cells exist, the authors have found liposome mediated transfection to be the most efficient. The document details the protocols and medium needed for efficient simple transfection of Spodoptera frugiperda cells.