Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Detection of Horizontal Gene Transfer by Natural Transformation in Native and Introduced Species of Bacteria in Marine and Synthetic Sediments.
Author Stewart, G. J. ; Sinigalliano, C. D. ;
CORP Author University of South Florida, St. Petersburg. Dept. of Marine Science.;Environmental Research Lab., Gulf Breeze, FL.;National Science Foundation, Washington, DC.
Publisher c1990
Year Published 1990
Report Number EPA-R-813589 ;NSF-OCE88-17172; EPA/600/J-92/219;
Stock Number PB92-195767
Additional Subjects Gene transfer ; Marine microorganisms ; Deoxyribonucleic acids ; Genetic transformation ; Rifampicin ; Drug resistance ; Sediments ; Reprints ; Pseudomonas stutzeri
Library Call Number Additional Info Location Last
NTIS  PB92-195767 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 08/22/1992
Collation 9p
Both naturally occurring marine sediments and artificial sediments were used as supports for natural transformation of marine bacteria. While transformation was not detected in cells of Pseudomonas stutzeri strain ZoBell suspended in artificial seawater, when recipient cells and rifampin resistant DNA were loaded onto sterile sediment columns, transformation could be detected at frequencies four to twenty times that for spontaneous resistance. Treatment of these columns with Dnase I reduced transformation frequencies to levels comparable to spontaneous resistance frequencies. Transformation was also detected when recipient cells and DNA were loaded on columns prepared from non-sterile sediments, although the frequencies of transformation were lower in these cases than when sterile sediments were employed. Results indicate that marine sediments facilitate the uptake and expression of exogenous DNA by transformable marine bacteria, and that sediments are a more likely niche for natural transformation than the water column in the marine environment.