Record Display for the EPA National Library Catalog

RECORD NUMBER: 42 OF 48

OLS Field Name OLS Field Data
Main Title Phylogenetically Based Studies of Microbial Ecosystem Perturbation.
Author Stahl, D. A. ;
CORP Author Illinois Univ. at Urbana-Champaign. Coll. of Veterinary Medicine.;Environmental Research Lab., Gulf Breeze, FL.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-88/354;
Stock Number PB89-237200
Additional Subjects Gastrointestinal system ; Cattle ; Monitoring ; Nucleic acids ; Reprints ; Biotechnology ; Microbial genetics ; Phylogeny ; Oligonucleotide probes ; 16S ribosomal RNA ; Monensin ; Microbial drug resistance
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB89-237200 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 12/18/1989
Collation 20p
Abstract
The use of comparative ribosomal RNA sequencing for studies in microbial ecology and microbial evolution is discussed. A specific study, using the 16S ribosomal RNA to monitor the bovine rumen microbial community, is described. The response of this community to antibiotic addition was evaluated by hybridization of the oligonucleotide probes to nucleic acid isolated from the rumen. The application of the ribosomal RNA based measure of microbial communities to general evaluations of community disruption, as might follow the release of genetically engineered microorganisms into the environment, is discussed. (Copyright (c) 1988 American Chemical Society.)