Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Method 1103.1: Escherichia coli (E. coli) in Water by Membrane Filtration Using Membrane-Thermotolerant Escherichia coli Agar (mTEC), July 2006.
CORP Author Environmental Protection Agency, Washington, DC. Office of Water.
Publisher Jul 2006
Year Published 2006
Report Number EPA/821/R-06/010;
Stock Number PB2007-101966
Additional Subjects Escherichia coli ; Membrane filtration ; Water sampling ; Bacteria ; Agar ; Enumeration ; Epidemiology ; Microorganisms ; Criteria ; Water quality ; Marine environment ; Health effects ; Gastroenteristis ; Feces ; Water pollution effects ; Surface waters ; Counting methods ; Calibration ; Growth(Biology) ; Contamination ; Quality control ; Pollution prevention ; Laboratories ; Test methods ;
Library Call Number Additional Info Location Last
NTIS  PB2007-101966 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 05/28/2007
Collation 45p
The MF method provides a direct count of bacteria in water based on the development of colonies on the surface of the membrane filter (Reference 18.4). A water sample is filtered through the membrane which retains the bacteria. After filtration, the membrane containing the bacterial cells is placed on a selective and differential medium, mTEC, incubated at 35DGC for 2 h to resuscitate injured or stressed bacteria, and then incubated at 44.5DGC for 22 h. Following incubation, the filter is transferred to a filter pad saturated with urea substrate. After 15 min, yellow, yellow-green or yellow-brown colonies are counted with the aid of a fluorescent lamp and a magnifying lens.