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RECORD NUMBER: 8 OF 10

OLS Field Name OLS Field Data
Main Title Molecular Regulation of the Induction of Cytochrome P-450E in the Estuarine Fish 'Fundulus heteroclitus'.
Author Kloepper-Sams, P. J. ;
CORP Author Woods Hole Oceanographic Institution, MA. ;Massachusetts Inst. of Tech., Cambridge.;National Science Foundation, Washington, DC.;Public Health Service, Washington, DC.;Environmental Protection Agency, Washington, DC.
Publisher cFeb 89
Year Published 1989
Report Number WHOI0-89-1; NSF-OCE83-10505 ;PHS-ES-4220;
Stock Number PB89-198204
Additional Subjects Estuaries ; Fishes ; Regulation ; Antibodies ; Theses ; Ribonucleic acid ; Cytochrome P-450 ; Enzyme induction ; Fundulus heteroclitus ; Xenobiotic metabolism
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NTIS  PB89-198204 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 12/18/1989
Collation 226p
Abstract
Regulation of induction of P450IA1 (P-450E) in teleosts was examined by investigating temporal relationships between P-450E protein, activity, and mRNA levels, and measuring protein and heme turnover in the teleost Fundulus heteroclitus. Monoclonal antibodies used for P-450E protein detection were specific in immunoblots for purified scup (Stenotomus chrysops) P-450E, a single band corresponding to P-450E in scup microsomal mixtures, and the xenobiotic-inducible orthologue in other fish including Fundulus. P-450E mRNA was measured by translation of total RNA, precipitation with anti-P-450E polyclonal antibodies and autoradiography, or by hybridization of RNA with a trout P450IA1 cDNA. P-450E and ethoxyresorufin O-deethylase activity rose coordinately after treatment with Beta-naphthoflavone, lagging behind mRNA increases by about 25 hours. mRNA levels declined rapidly, despite prolonged elevated protein and activity levels. In a dual label experiment, P-450E was precipitated from solubilized microsomes. The apoprotein was calculated to have a half-life of 32 to 43 hours, the heme moiety a longer half-life of 104 hours. These results support a hypothesis that transcriptional enhancement is involved in initial stages of P-450E induction, while other forms of control are important in maintenance of P-450E expression. The study addressed a specific chemico-biological interaction - the organism's biochemical response to a challenge by foreign compounds - which occurs in the marine environment. (Copyright (c) Pamela J. Kloepper-Sams, 1989.)