Cadmium and zinc toxicity to the flagfish (Jordanella floridae) was determined on the basis of 96-hr median lethal concentrations (LC50) and significant decreases (P=0.05) in survival, growth, and reproduction over the complete life cycle of the fish. The 96-hr LC50 values for cadmium and zinc to juvenile flagfish were 2,500 and 1,500 microg/liter, respectively. In chronic tests, reproduction was the most sensitive indicator of cadmium toxicity and was inhibited at 8.1 microg/liter. Tissue-concentration analysis showed that fish exposed to concentrations of 1.7 microg/liter and above accumulated significantly greater amounts of cadmium than those in the controls. In zinc tests, survival of larvae (not exposed as embryos) and growth of females were the most sensitive measure of zinc toxicity and were reduced at respective concentrations of 85 and 51 microg/liter.