Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Methods for Spawning, Culturing and Conducting Toxicity Tests with Early Life Stages of Estuarine and Marine Fishes.
Author Middaugh, D. P. ; Goodman, L. R. ; Hemmer., M. J. ;
CORP Author Environmental Research Lab., Gulf Breeze, FL.
Publisher 1994
Year Published 1994
Report Number EPA/600/A-94/034;
Stock Number PB94-155389
Additional Subjects Marine fishes ; Toxicity ; Estuaries ; Tests ; Life cycles ; Geographical distribution ; Minnows ; Algae ; Shrimps ; Toxic tolerances ; Environmental effects ; Evaluation ; Assessments ; Foreign countries ; Cyprinodon variegatus ; Spawning ; Sheepshead minnow
Library Call Number Additional Info Location Last
NTIS  PB94-155389 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 09/01/1994
Collation 26p
The chapter provides a detailed description of the life history, geographical distribution of the life history, geographical distribution, and procedures for laboratory spawning, culturing and testing of five fishes, the sheepshead minnow, Cyprinodon variegatus, the inland silverside, Menidia beryllina, Atlantic silverside, M. menidia, California grunion, Leuresthes tenuis, and topsmelt, Atherinops affinis. Procedures for conducting acute toxicity tests (static and flow-through) as well as early life-stage toxicity tests are presented. Methods required for culturing of food organisms, the alga, Isochrysis galbana, rotifers, Brachionus plicatilus, and brine shrimp, Artemia salina, are also described. Tabular and diagrammatic data summaries of pertinent information required for utilization of each species in evaluation of environmental toxicants is presented.