Record Display for the EPA National Library Catalog

RECORD NUMBER: 9 OF 13

OLS Field Name OLS Field Data
Main Title Fluorometric Determination of the DNA (Deoxyribonucleic Acid) Concentration in Municipal Drinking Water.
Author McCoy, W. F. ; Olson, B. H. ;
CORP Author California Univ., Irvine.;Environmental Protection Agency, Cincinnati, OH. Water Engineering Research Lab.
Year Published 1985
Report Number EPA-R-809817; EPA/600/J-85/466;
Stock Number PB87-170460
Additional Subjects Deoxyribonucleic acids ; Bacteria ; Water pollution ; Fluorometers ; Municipalities ; Concentration(Composition) ; Turbidity ; Collecting methods ; Chlorine ; Assaying ; Cells(Biology) ; Reprints ; Drinking water
Holdings
Library Call Number Additional Info Location Last
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Status
NTIS  PB87-170460 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 8p
Abstract
DNA concentrations in municipal drinking water samples were measured by fluorometry, using Hoechst 33258 fluorochrome. The concentration, extraction, and detection methods used were adapted from existing techniques. The method is reproducible, fast, accurate, and simple. The amounts of DNA per cell for five different bacterial isolates obtained from drinking water samples were determined by measuring DNA concentration and total cell concentration (acridine orange epifluorescence direct cell counting) in stationary pure cultures. The relationship between DNA concentration and epifluorescence total direct cell concentration in 11 different drinking water samples was linear and positive; the amounts of DNA per cell in these samples did not differ significantly from the amounts in pure culture isolates. (Copyright (c) 1985, American Society for Microbiology.)