Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Screening methods for toxic substances /
Author Sikka, Harish Chandra.
CORP Author Syracuse Research Corp., N.Y.;Health Effects Research Lab., Research Triangle Park, N.C. Environmental Toxicology Div.
Publisher U.S. Environmental Protection Agency, Health Effects Research Laboratory ,
Year Published 1978
Report Number EPA-600/1-78-014; EPA-68-02-1786
Stock Number PB-277 017
OCLC Number 42843379
Subjects Toxicity testing. ; Neurotoxic agents. ; Phenytoin--Toxicology. ; DDT (Insecticide)--Toxicology. ; Neurotoxic agents--Screening
Additional Subjects Brain ; Neurology ; Toxicology ; DDT ; Pesticides ; Synapses ; Nervous system ; Nerve cells ; Membranes ; Permeability ; Ions ; Pathology ; Detection ; Toxicity ; Tests ; Sublethal dosage ; Rats ; Laboratory animals ; In vitro analysis ; Toxic substances ; Synaptosomes ; Fluorescent probes
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EJBD ARCHIVE EPA 600-1-78-014 Headquarters Library/Washington,DC 05/27/2014
EJBD  EPA 600-1-78-014 c.1 Headquarters Library/Washington,DC 06/09/2014
EJED  EPA 600/1-78/014 OCSPP Chemical Library/Washington,DC 01/13/2001
EKAM  EPA 600/1-78/014 Region 4 Library/Atlanta,GA 11/19/1999
EKBD  EPA-600/1-78-014 Research Triangle Park Library/RTP, NC 02/23/2000
ELDD  EPA 600/1-78/014 NHEERL/MED Library/Duluth,MN 09/08/2000
NTIS  PB-277 017 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/23/1988
Collation v, 18 p. ; 28 cm.
The present report deals with the application of fluorescent probe technique to the detection of conformational changes in rat brain synaptosomes under the influence of certain neurotoxic chemicals, chlorophenothane (DDT) and diphenylhydantoin, which are expected to produce functional changes in nerve membranes. Pretreatment of the rats with (1) a single dose of DDT capable of producing neurological symptoms and (2) repeated sub-lethal doses of DDT did not alter the fluorescence characteristics of the hydrophobic probe 8-anilino-1-naphthalene sulfonic acid (ANS) bound to the synaptosomes. DDT treatment also showed no effect on native protein fluorescence of synaptosomes. Exposure of synaptosomes to diphenylhydantoint in vitro did not induce conformational changes as measured by ANS and intrinsic fluorescence.
"EPA-600/1-78-014". EPA contract no. 68021786; EPA project officer: Larry L. Hall. Includes bibliographical references (p. 16-17).