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RECORD NUMBER: 7 OF 10

OLS Field Name OLS Field Data
Main Title Kinetochore-Staining of Spermatid Micronuclei: Studies of Mice Treated with X-Radiation or Acrylamide.
Author Collins, B. W. ; Howard, D. R. ; Allen, J. W. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
Publisher c1992
Year Published 1992
Report Number EPA/600/J-92/193;
Stock Number PB92-188994
Additional Subjects X rays ; Acrylamides ; Spermatozoa ; Micronuclei ; Mice ; Fluorescent antibody technique ; Meiosis ; Staining ; Reprints ; Kinetochores
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB92-188994 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 08/22/1992
Collation 10p
Abstract
The rodent spermatid micronucleus (MN) assay was used in conjunction with immunofluorescent techniques to distinguish kinetochores in MN following exposure of mice to X-radiation or acrylamide. After either treatment, modest increases in kinetochore-positive MN were observed. Spermatids which had been exposed during meiotic prophase to X-rays (400 cGy) had approximately 10-fold increases in MN compared to controls; up to 15% of the MN observed were kinetochore-positive. Following acrylamide treatment of meiotic prophase cells, there was a doubling of spermatid MN over baseline levels, approximately one-third of which were kinetochore-positive. (Copyright (c) 1992 Elsevier Science Publishers B.V.)