Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Use of Immunofluorescence and Phase-Contrast Microscopy for Detection and Identification of 'Giardia' Cysts in Water Samples.
Author Sauch, J. F. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1985
Report Number EPA/600/J-85/365;
Stock Number PB86-177771
Additional Subjects Cysts ; Disease vectors ; Surface waters ; Water pollution ; Monitoring ; Reprints ; Giardiasis ; Fluorescent antibody technique ; Waterborne diseases
Library Call Number Additional Info Location Last
NTIS  PB86-177771 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 6p
A method was developed in which indirect immunofluorescence and phase-contrast microscopy are used for rapid detection and identification of Giardia cysts in raw and finished water supplies. When anti-Giardia cyst antiserum and fluorescein conjugate were applied to known Giardia cysts on membrane filters, the cysts fluoresced bright green when they were illuminated by UV light. This procedure permitted individual cysts to be quickly located even in samples heavily contaminated with other microorganisms and debris. The identity of presumptive Giardia cysts located in this way could then be confirmed by observing characteristic internal morphological features with phase-contrast microscopy. With this method, Giardia cysts were detected and their identities were confirmed in samples taken from raw and finished surface water supplies during several recent outbreaks.