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RECORD NUMBER: 85 OF 309

OLS Field Name OLS Field Data
Main Title Enumeration of 'Vibrio vulnificus' on Membrane Filters with a Fluorescently Labeled Oligonucleotide Probe Specific for Kingdom-Level 16S rRNA Sequences.
Author Heidelberg, J. F. ; O'Neill, K. R. ; Jacobs, D. ; Colwell, R. R. ;
CORP Author Maryland Univ., College Park.;Environmental Research Lab., Gulf Breeze, FL.
Publisher c1993
Year Published 1993
Report Number EPA/600/J-94/004;
Stock Number PB94-140761
Additional Subjects 16S ribosomal RNA ; Microbial colony count ; Artificial membranes ; Oligonucleotide probes ; Fluorescent dyes ; Base sequence ; Fluorescence microscopy ; Nucleic acid hybridization ; Reprints ; Vibrio vulnificus
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB94-140761 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 05/14/1994
Collation 5p
Abstract
Vibrio vulnificus was enumerated on membrane filters after hybridization with a fluorescent oligonucleotide eubacterial probe. Cells were hybridized in liquid buffer or directly on membrane filters. There was no significant difference between fluorescent oligonucleotide direct counts and acridine orange direct counts (P > 0.05). Liquid buffer hybridization was preferable to direct filter hybridization. (Copyright (c) 1993, American Society for Microbiology.)