Record Display for the EPA National Library Catalog

RECORD NUMBER: 35 OF 308

OLS Field Name OLS Field Data
Main Title Characterization of Normal Human Lung Lymphocytes and Interleukin-2-Induced Lung T Cell Lines.
Author Becker, S. ; Harris, D. T. ; Koren, H. S. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;ABB Environmental Services, Inc., Chapel Hill, NC.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/527;
Stock Number PB91-213504
Additional Subjects Lung ; T lymphocytes ; Cell line ; Interleukin-2 ; Humans ; Bronchoalveolar lavage fluid ; Monoclonal antibodies ; Phenotype ; HLA-DR antigens ; Cytotoxic T lymphocytes ; CD antigens ; Immunoglobulins ; Mitogens ; Flow cytometry ; Fluorescent antibody techniques ; Fluorescence ; Reprints ;
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB91-213504 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 11/26/1991
Collation 12p
Abstract
Lymphocytes from the lower respiratory tract were obtained by bronchoalveolar lavage of healthy, non-smoking individuals. Various monoclonal antibodies characterizing activated T cells, helper-inducer and suppressor-inducer T cell subsets, and naive versus memory cells were used to define the phenotype of these lymphocytes. The highly variable CD4/CD8 ratio (0.3 to 6.6; mean=2.1) and the large proportion of the T cells expressing HLA-DR (9 to 38%; mean=21%) sugested that the T cells were recently activated by antigens selectively stimulating either helper or cytotoxic/suppressor T cell function. The majority (>70%) of the CD4+ helper T cells was CD45RO+ CD45RA-, a phenotype found on T cells that provide help for B cell immunoglobulin synthesis. Lung T cells proliferated poorly in response to phytohemagglutinin and concanavalin A but did respond to activation with low concentrations of anti-CD3 mAB (2 to 25 ng/ml) and to interleukin-2 (IL-2) alone to similar extent as did autologous peripheral blood lymphocytes.