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RECORD NUMBER: 273 OF 309

OLS Field Name OLS Field Data
Main Title Separation of the Isomeric Phenols of Benzo(a)Pyrene by High Performance Liquid Chromatography.
Author Elnenaey, Elsayed A. ; Schoor, W. Peter ;
CORP Author Environmental Research Lab., Gulf Breeze, FL.
Year Published 1981
Report Number EPA-600/J-80-125;
Stock Number PB81-233637
Additional Subjects Phenols ; Environmental surveys ; Aromatic polycyclic hydrocarbons ; Separation ; Fluorescence ; Ultraviolet spectroscopy ; Reprints ; Benzopyrenes ; High performance liquid chromatography
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB81-233637 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/23/1988
Collation 10p
Abstract
A technique has been developed to separate a mixture of the twelve isomeric phenols of benzo(a)pyrene by using High-Performance Liquid Chromatography with fluorescence detection and various sequences of methanol/water gradients. Detection by fluorescence has increased the sensitivity of the method when compared with UV detection. The effects of flow rate, temperature, and various types of gradients on the separation of the isomeric phenols are discussed. It has been found that under the conditions normally employed to separate these phenols from other metabolites of benzo(a)pyrene, several of the phenols are not separated possibly leading to incorrect identification as well as quantitation. Diols and diones of benzo(a)pyrene are also separated by this procedure.