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RECORD NUMBER: 260 OF 309

OLS Field Name OLS Field Data
Main Title Reversibility of Tributyltin-Induced Toxicity In vitro as a Function of Concentration and Duration of Exposure (C X T).
Author Zucker, R. W. ; Massaro, E. J. ; Elstein, K. H. ;
CORP Author NSI Technology Services Corp., Research Triangle Park, NC. ;ManTech Environmental Technology, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC. Developmental Toxicology Div.
Publisher c1992
Year Published 1992
Report Number EPA-68-02-4450; EPA/600/J-92/115;
Stock Number PB92-164854
Additional Subjects Toxicity ; Dose-response relationships ; Pesticides ; Cell membrane permeability ; Exposure ; Cytoplasm ; Fluorescence ; S phase ; Cell cycle ; Acute erythroblastic leukemia ; Deoxyribonucleic acids ; Reprints ; Tributyltin
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB92-164854 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 08/22/1992
Collation 12p
Abstract
The toxicity exhibited by murine erythroleukemic cells (MELC) exposed to tributyltin (TBT) is a function of both concentration (C) and duration of exposure (T). At or above a critical C x T product value (CPV), exposed MELC exhibit severe, irreversible toxicity: decreased membrane integrity (viability, measured by propidium iodide (PI) exclusion), grossly perturbed cell cycle distributions, and fixation of the plasma membrane/cytoplasm complex. Below the CPV, exposed cells exhibit retention of carboxyfluorescein (CF) fluorescence (indicative of decreased plasma membrane permeability) and decreased cell proliferation, a result of retardation of progression into, through, and out of the S (DNA synthetic) phase of the cell cycle. However, following washout and recovery, mean CF fluorescence, cell proliferative capacity, and cell-cycle kinetics return to control levels. These results suggest that the toxic changes induced by TBT exposure may be reversible if exposure conditions do not exceed the CPV.