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RECORD NUMBER: 115 OF 309

OLS Field Name OLS Field Data
Main Title Fiber Optic Fluoroimmunosensor for the Detection of Polychlorinated Biphenyls.
Author Rogers, K. R. ; Van Emon, J. M. ; Kline, R. ; Wright, J. ; Zhao, Q. ;
CORP Author Maryland Univ. at Baltimore.;Environmental Monitoring Systems Lab., Las Vegas, NV.
Publisher Feb 95
Year Published 1995
Report Number EPA-CR-820460; EPA/600/A-95/058;
Stock Number PB95-218855
Additional Subjects Fluorometers ; Chlorinated organic compounds ; DNA adducts ; Reprints ; Fiber optics ; Technology innovation ; Analytical chemistry ; Field tests ; Optical measuring instruments ; Fluorescence spectroscopy ; Immunoassay ; Pollution monitoring ; Hazardous materials ; Toxic substances ; Fluoroimmunosensor ; PCBs(Polychlorinated biphenyls) ; Anti-PCB serum ; Evanescent wave fluorometer
Holdings
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Status
NTIS  PB95-218855 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 09/02/1995
Collation 10p
Abstract
There currently exists a critical and growing need for cost-effective, real-time, and in situ methods for detection of environmental pollutants such as polychlorinated biphenyls (PCBs). Herein reported is a evanescent wave Fluoroimmununosensor for measurement of PCBS. 2,4-5-Trichlorophenoxybutyric acid (TCPB) was cross linked to fluorescein ethyleneamine (TCPB-FL) and used as a optical signal generator. Protein A purified IgG, obtained from anti-PCB serum, was immobilized on quartz fibers and used as the biological recognition element in an evanescent wave fluorimeter. The optical signal was generated by the binding of the fluorescent probe TCPB-FL to the antibody (Ab)-coated fiber. Fluorescent probe concentrations in the nM range resulted in reversible binding. Several Aroclors displaced the fluorescent probe (TCPB-FL) in a concentration-dependent manner. Arochlor 1248 was detected from the ppb to ppm range. Arochlor 1242 was also measured in methanol extracts from standard reference material soils.