||Membrane Filter-Fluorescent Antibody Method for Detection and Enumeration of Bacteria in Water. Rapid Identification of Microbial Populations in Water.
Guthri, Rufus K. ;
Reede, Dennis J. ;
||North Texas State Univ., Denton. Dept. of Biology.
( Bacteria ;
Water analysis) ;
Quantitative analysis ;
Water quality ;
Fluid filters ;
Escherichia coli ;
Fluorescent antibody techniques
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A technique which employs use of non-fluorescing membrane filters and specific FITC-labelled antiserum has been successfully used in the identification and enumeration of known species of E. coli which have been added to natural populations of bacteria found in water. The quantitative results compared favorably with tests described in the standard methods. The use of a dissecting microscope with an external lighting arrangement provided a simple requirement for equipment. This method appears useful in monitoring specific bacterial types from waters which were being monitored for specific pollution. (Author)