Record Display for the EPA National Library Catalog

RECORD NUMBER: 371 OF 3914

OLS Field Name OLS Field Data
Main Title Automated Biochemical Identification of Bacterial Fish Pathogens Using the Abbott Quantum II.
Author Teska, J. D. ; Shotts, E. B. ; Hsu, T. ;
CORP Author Georgia Univ., Athens. Coll. of Veterinary Medicine. ;Rhone Merieux, Inc., Athens, GA.;Environmental Research Lab., Athens, GA. Office of Research and Development.
Publisher c1989
Year Published 1989
Report Number EPA-R-812638; EPA/600/J-89/026;
Stock Number PB90-100827
Additional Subjects Enterobacteriaceae ; Fishes ; Test equipment ; Aeromonas ; Salmonella ; Serratia ; Reprints ; Abbott Quantum III ; Diagnostic uses ; Feasibility studies ; Yersinia ; Edwardsiella
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB90-100827 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 03/10/1990
Collation 7p
Abstract
The Quantum II was originally designed by Abbott Diagnostics for automated rapid identification of members of Enterobacteriaceae. The study was initiated to determine the feasibility of expanding its use to include identification of bacterial fish pathogens. Ten to fifty isolates of Edwardsiella ictaluri, Serratia liquefaciens, Yersinia rukert, Aeromonas hydrophila, typical Aeromonas salmoncida, and atypical Aeromonas salmoncida were utilized to determine optimal incubation conditions, relative stability of the biochemicals, and ability to obtain consistent biocide numbers. After sorting the octal biocides from the 169 isolates into groupings using a cluster analysis technique, it was shown by a Chi-square goodness of fit test that isolates of a given species were sorted into the same cluster group at a frequency of at least 99%. Results of the study illustrate the usefulness of the Quantum II BID system for the identification of bacterial fish pathogens not contained in the system's memory module. (Copyright (c) Wildlife Disease Association 1989.)