Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Enzyme-based detection of chlorinated hydrocarbons in water /
Author Offenhartz, Barbara H. ; Lefko, J. L.
Other Authors
Author Title of a Work
Lefko, Janet L.
CORP Author JRB Associates, Inc., McLean, VA. ;B and M Technological Services, Inc., Cambridge, MA.;Environmental Protection Agency, Edison, NJ. Hazardous Waste Engineering Research Lab.
Publisher U.S. Environmental Protection Agency, Hazardous Waste Engineering Research Laboratory,
Year Published 1985
Report Number EPA/600/2-85/048; EPA-68-03-3113
Stock Number PB85-191716
OCLC Number 48158053
Subjects Organochlorine compounds. ; Hydrocarbons. ; Water--Pollution--United States--Measurement. ; Pesticides--Environmental aspects. ; Water--Pollution--Measurement.
Additional Subjects Water analysis ; Chlorine organic compounds ; Hazardous materials ; Monitors ; Soil analysis ; Catalysis ; Field tests ; Water quality ; pH ; Concentration(Composition) ; Cost effectiveness ; Performance evaluation ; Enzymes ; Pesticides ; Sampling ; Water pollution detection ; LDH method ; Immobilized enzymes ; Solid wastes ; Polychlorinated biphenyls
Library Call Number Additional Info Location Last
NTIS  PB85-191716 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 01/01/1988
Collation 51 pages : illustrations ; 28 cm
An enzyme-based approach for detecting hazardous levels of high molecular weight chlorinated hydrocarbons in natural waters has been explored. An extensive review of the literature indicated that the enzymes, lactate dehydrogenase, carbonic anhydrase, hexokinase, phosphorylase and an ATPase are suitable for field method development. Lactate dehydrogenase (LDH) was chosen to develop a method that promises rapid, reliable and cost-effective detection. The LDH catalyzed reaction is used to detect chlorinated hydrocarbons, which reduce the rate of the reaction by reversibly inhibiting the enzyme. The analysis uses pH detection and takes 5 minutes. So far, the LDH method has been used to detect aldrin, toxaphene, DDT, PCBs, pentachlorophenol and 2,4,5,-T at the parts per million level. A review of the literature suggests that most organochlorine pesticides, PCBs, polychlorinated phenols and chlorophenoxy derivatives can be detected by the LDH method. Preliminary investigations of potential interferants suggest that the LDH method selectively detects chlorinated hydrocarbons. Work was begun on immobilizing the enzyme for incorporation in water monitor and field detector designs. The LDH method shows potential for use in field devices and for processing extracts of soils and wastes, as well as water samples. These method adaptations are recommended for future work.
Caption title. "April 1985." "EPA/600/2-85/048." Microfiche.