Record Display for the EPA National Library Catalog

RECORD NUMBER: 6 OF 29

OLS Field Name OLS Field Data
Main Title Effects of a Cytokinetic Block on the Determination of Micronucleus Induction in Chinese Hamster Lung Fibroblasts.
Author Mueller, C. ; Anderson, S. C. ;
CORP Author Environmental Research Lab., Narragansett, RI. ;Science Applications International Corp., Narragansett, RI.
Publisher c1989
Year Published 1989
Report Number ERLN-X159 ;EPA/600/J-89/262;
Stock Number PB90-196635
Additional Subjects Lung ; Mutagens ; In vitro analysis ; Mutations ; Reprints ; Micronucleus test ; Fibroblasts ; Cell cycle ; Chinese hamsters ; Mitomycin C ; Cytochalasin B
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB90-196635 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/15/1990
Collation 6p
Abstract
Approximately 63,000 chemicals are found in our foods, drugs, consumer products and environment. There is proof that human exposure to some of these chemicals can have carcinogenic, teratogenic and mutagenic effects. Several standard short-term tests are routinely employed to test for the occurrence of these mutagenic conditions. They include chromosomal breakage and rearrangement (clastogenicity) assays, gene mutation assays, and cell death assays (reduction in mitotic index or cell colonies). Chromosomal aberrations are a common cell response to mutagenic exposure. Recently the micronucleus test has gained popularity as a more rapid, less expensive method with which to detect this type of damage. Mutagenic exposure in dividing cells may cause chromosome damage producing acentric chromatid or chromosome fragments. Treatment of these cells with a known mutagenic and clastogenic compound, with the addition of a cytokinetic block, will determine if this cell line can be used for the detection and determination of micronuclei.