||Measurement of Methanotroph and Methanogen Signature Phosopholipids for Use in Assessment of Biomass and Community Structure in Model Systems.
Nichols, P. D. ;
Mancuso, C. A. ;
White, D. C. ;
||Florida State Univ., Tallahassee. Dept. of Biological Science. ;Commonwealth Scientific and Industrial Research Organization, Hobart (Australia). Div. of Oceanography. ;Tennessee Univ., Knoxville. Inst. for Applied Microbiology.;Robert S. Kerr Environmental Research Lab., Ada, OK.
Natural gas ;
Fatty acids ;
Oxidation reduction reactions ;
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Methanotrophic biomass and community structure were assessed for a soil column enriched with natural gas. An increase in microbial biomass, based on phospholipid ester-linked fatty acids (PLFA), was apparent for the natural gas-enriched column relative to a control column and untreated surface soil. Following GC-MS analyses of the derivatized monounsaturated fatty acids, the major component (22 percent of the PLFA) of the natural gas-enriched column was identified as 18:1 delta 10C. The relatively novel fatty acid has only been previously reported as a major component in methanotrophs. Its presence in the soil, together with other supportive evidence, implies that the microbial metabolic group makes a large contribution to the column flora. Other microbial groups were also recognized and differences compared between the soils analyzed. (Copyright (c) 1987 Pergamon Journals Ltd.)