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RECORD NUMBER: 218 OF 799

OLS Field Name OLS Field Data
Main Title Detection and Comparison of DNA Adducts After In vitro and In vivo Diesel Emission Exposures.
Author Gallagher, J. ; George, M. ; Kohan, M. ; Thompson, C. ; Shank, T. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;National Inst. of Environmental Health Sciences, Research Triangle Park, NC. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
Publisher c1993
Year Published 1993
Report Number EPA/600/J-93/359;
Stock Number PB93-228856
Additional Subjects DNA adducts ; Exhaust gases ; Diesel fuels ; Aromatic polycyclic hydrocarbons ; Environmental pollutants ; In vivo analysis ; In vitro analysis ; Xanthine oxidase ; Comparison ; Rodents ; Humans ; Reprints ;
Holdings
Library Call Number Additional Info Location Last
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Status
NTIS  PB93-228856 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 11/22/1993
Collation 6p
Abstract
Development of methodologies to evaluate certain classes of polycyclic aromatic compounds (PAC) detected in complex mixtures to which humans are exposed would greatly improve the diagnostic potential of (32)P-postlabeling analysis. Identification of DNA adduct patterns of specific exposure-related marker adducts would strengthen associations between observed DNA adducts and exposures to different environmental pollutants (e.g., kerosene, cigarette smoke, coke oven, and diesel). Diesel-modified DNA adduct patterns were compared in various in vitro and in vivo rodent model systems and then compared to DNA reactive oxidative and reductive metabolites of 1-nitropyrene. The formation of nitrated-polycyclic aromatic hydrocarbon (nitrated-PAH) DNA adducts, derived from the metabolism of diesel extract constituents, was enhanced relative to other PAH-derived DNA adducts via xanthine oxidase-catalyzed nitroreduction. These adducts were detectable only by the butanol extraction version of the postlabeling analysis. Marker adducts detected in the various test systems presented here will assist in characterizing nuclease-P1-sensitive nitrated PAH adducts in humans.