Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title New Method for Excystation of Giardia.
Author Sauch, J. F. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1987
Report Number EPA/600/D-87/046;
Stock Number PB87-145918
Additional Subjects Giardia ; Cysts ; In vitro analysis ; Peptones ; Agents ; Trypsin ; Enzymes ; Microbiology ; Excystation ; Antigenic analysis ; Protease peptone ; Phytone peptone ; Hanks Balanced Salt Solution(HBSS) ; Giardia lamblia ; Giardia muris ; Electrophoretic analysis
Library Call Number Additional Info Location Last
NTIS  PB87-145918 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/21/1988
Collation 21p
In vitro excystation of Giardia is used to estimate cyst viability and may also be used to obtain trophozoites and cyst walls for analysis. Recently published excystation procedures include the use of trypsin, serum or bile salts in the excystation step. Trypsin, serum or bile salts in the excystation medium was replaced by proteose peptone for G. muris and phytone peptone for G. lamblia. Comparison between this method utilizing peptones and that of Schaefer utilizing trypsin showed no significant differences in percent excystation for both G. muris and G. lamblia.