Record Display for the EPA National Library Catalog

RECORD NUMBER: 4 OF 4

OLS Field Name OLS Field Data
Main Title The Dynamics of an estuary as a natural ecosystem, II /
Author Koch, Robert B. ; Vernberg, F. J. ; Kitchens, W. ; McKellar, H. ; Summers, K. ; Bonnell, R.
Other Authors
Author Title of a Work
Vernberg, F. John,
CORP Author Mississippi State Univ., Mississippi State. Dept. of Biochemistry.;Environmental Research Lab., Gulf Breeze, FL.
Publisher U.S. Environmental Protection Agency, Office of Research and Development, Environmental Research Laboratory ; National Technical Information Service [distributor],
Year Published 1978
Report Number EPA-600/3-78-092; EPA-R-804407-01
Stock Number PB-289 969
OCLC Number 08081667
Subjects Estuarine ecology--South Carolina. ; Estuarine ecology--Mathematical models. ; Salt marsh ecology--South Carolina. ; Salt marsh ecology--Mathematical models. ; Tidemarsh ecology--South Carolina ; Tidemarsh ecology--Mathematical models
Additional Subjects Pesticides ; Antibodies ; Development ; Chlorine organic compounds ; Inhibition ; Recommendations ; Rabbits ; Chickens ; Laboratory animals ; Antigen antibody reactions ; Enzymes ; Insecticides ; Chlorine organic compounds ; Kelevan ; Adenosine triphosphatase ; Kepone ; Immune serums ; Methenocyclobutapentalene levulinic acid/decachlorooctahydro-hydroxy-(ethyl-ester) ; Cyclobutapentalenone/decachlorocathydro ; North Inlet Estuary ; Ecology ; Water pollution ; Mathematical models ; Aquatic plants ; Aquatic animals ; Plankton ; Intertidal zone ; Marine microorganisms ; Invertebrates ; Food chains ; Primary biological productivity ; Fishes ; Birds ; Respiration ; Biomass ; Nutrients ; Computer programming ; South Carolina ; Spartina alterniflora ; Salt marshes ; Baseline studies
Internet Access
Description Access URL
https://nepis.epa.gov/Exe/ZyPDF.cgi?Dockey=9101XGX1.PDF
http://nepis.epa.gov/Exe/ZyPDF.cgi?Dockey=9101XGZG.PDF
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
EJAD  EPA 600/3-78-092 Region 3 Library/Philadelphia, PA 03/19/1993
EJBD ARCHIVE EPA 600-3-78-092 Headquarters Library/Washington,DC 11/13/2013
EJBD  EPA 600-3-78-092 c.1 Headquarters Library/Washington,DC 03/24/2014
EJDD  EPA 600/3-78-092 OASQA Library/Fort Meade,MD 03/31/1995
EKAM  QP82.2.P6E2 600, 1978, no.092 Region 4 Library/Atlanta,GA 05/13/1994
EKCD  EPA-600/3-78-092 NHEERL/GED Library/Gulf Breeze,FL 07/21/2015
ERAD  EPA 600/3-78-092 Region 9 Library/San Francisco,CA 10/22/2012
ESAD  EPA 600-3-78-092 Region 10 Library/Seattle,WA 08/23/2017
NTIS  PB-289 963 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/23/1988
NTIS  PB-289 969 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/23/1988
Collation viii, 76 pages : illustrations ; 28 cm.
Abstract
The report describes development of an antibody to an organochlorine pesticide to be used in studies related to its inhibition of the ATPase system. Kelevan, the condensation product of ethyl levulinate and Kepone, was successfully conjugated to bovine serum albumin (BSA), fibrinogen (BF), and gamma globulin (BGG). Rabbits and chickens preimmunized with BSA and then immunized with BSA-Kelevan produced antibodies to both the hapten, Kelevan, and the carrier protein BSA. Antiserum to Kelevan protected ATPase activity against Kepone and its derivatives. The titer of antibody to Kelevan was critical since antiserum with only trace amounts of Kelevan antibody failed to protect the ATPase activity against Kepone Inhibition. Antibody was concentrated by Na2S04 fractional precipitation of the antiserum and obtained in pure form by affinity chromatography using BGG-Kel covalently linked to Sepharose 4B. Pure antibody was obtained from untreated blood serum or plasma with no prior pretreatment or fractionation using the BGG-Kel affinity column. Complete protection of mitochondrial mg2+ATPase activity from in vitro inhibition of Kepone was obtained using a 1.2 mg quantity of na2S04 fractionated antibody and only 120 micrograms of pure antibody. Reversal of ATPase inhibition was readily obtained by addition of antibody prior to addition of substrate to the reaction mixture.
Notes
"Belle W. Baruch Institute for Marine Biology and Coastal Research, University of South Carolina. "October 1978." Includes bibliographical references (pages 72-76).