Record Display for the EPA National Library Catalog
RECORD NUMBER: 3 OF 3
|OLS Field Name||OLS Field Data|
|Main Title||Method 1604 / total coliforms and Escherichia coli in water by membrane filtration using simultaneous detection technique (MI Medium).|
|CORP Author||Environmental Protection Agency, Washington, DC. Office of Water.|
|Publisher||Environmental Protection Agency, Office of Water,|
|Report Number||EPA 821-R-02-024|
|Subjects||Escherichia coli--United States--Prevention ; Enterobacteriaceae--United States.|
|Additional Subjects||Coliforms ; Escherichia coli ; Bacteria ; Water sampling ; Membrane filtration ; Enumeration ; Drinking water ; Detection ; Feces ; Water pollution effects ; Water quality ; Contamination ; Calibration ; Pollution prevention ; Microbial analysis ; Quality control ; Laboratories ; Test methods|
|Collation||14 pages : illustrations ; 28 cm|
This test method describes a sensitive and differential membrane filter (MF) medium, using MI agar or MI broth, for the simultaneous detection and enumeration of both total coliforms (TC) and Escherichia coli (E. coli) in water samples in 24 hours or less on the basis of their specific enzyme activities. Two enzyme substrates, the fluorogen 4-Methylumbelliferyl- b-D-galactopyranoside (MUGal) and a chromogen Indoxyl-b-D-glucuronide (IBDG), are included in the medium to detect theenzymes b-galactosidase and b-glucuronidase, respectively, produced by TC and E. coli, respectively. Total coliforms include species that may inhabit the intestines of warm-blooded animals or occur naturally in soil, vegetation, and water. They are usually found in fecally-polluted water and are often associated with disease outbreaks. Although they arenot usually pathogenic themselves, their presence in drinking water indicates the possible presence of pathogens. E. coli, one species of the coliform group, is always found in feces and is, therefore, a more direct indicator of fecal contamination and the possible presence of enteric pathogens.
EPA 821-R-02-024. September 2002. Cover title. Includes bibliographical references.
Scope and application -- Summary of method -- Definitions -- Interferences and contamination -- Safety -- Equipment and supplies -- Reagents and standards -- Sample collection, preservation, and storage -- Calibration and standardization -- Quality control (QC) -- Procedure -- Data analysis and calculations -- Method performance -- Pollution prevention -- Waste management -- References -- Tables and figures.