Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Method 1603 : Escherichia coli (E. coli) in water by membrane filtration using modified membrane-thermotolerant Escherichia coli agar (modified mTEC).
CORP Author Environmental Protection Agency, Washington, DC. Office of Water.
Publisher United States, Environmental Protection Agency, Office of Water,
Year Published 2002
Report Number EPA/821/R-02/023
Stock Number PB2003-100128
Subjects Escherichia coli--United States--Prevention.
Additional Subjects Escherichia coli ; Membrane filtration ; Water sampling ; Bacteria ; Agar ; Epidemiology ; Water pollution effects ; Marine environment ; Fresh water ; Health effects ; Gastrointestines ; Feces ; Substrates ; Surface waters ; Water quality ; Counting methods ; Calibration ; Contamination ; Laboratories ; Test methods
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
NTIS  PB2003-100128 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 01/01/1988
The method describes a revised Escherichia coli membrane filter (MF) procedure, a single-step method that uses one medium, modified mTEC Agar, and does not require the transfer of the membrane filter to another medium or other substrate. The modified medium contains a chromogen (5-bromo-6-chloro- 3-indolyl--D-glucuronide), which is catabolized to glucuronic acid and a red- or magenta-colored compound byE. coli that produce the enzyme D-glucuronidase. The apparatus and equipment, and sampling, filtration, and verification procedures for this modified mTEC method are identical to those of the original mTEC method. Because the bacterium is a natural inhabitant only of the intestinal tract of warm-blooded animals, its presence in water samples is an indication of fecal pollution and the possible presence of entericpathogens.
"September 2002." "EPA-821-R-02-023." Includes bibliographical references. Microfiche.