Record Display for the EPA National Library Catalog
RECORD NUMBER: 2 OF 3
|OLS Field Name||OLS Field Data|
|Main Title||Method 1603 : Escherichia coli (E. coli) in water by membrane filtration using modified membrane-thermotolerant Escherichia coli agar (modified mTEC).|
|CORP Author||Environmental Protection Agency, Washington, DC. Office of Water.|
|Publisher||United States, Environmental Protection Agency, Office of Water,|
|Subjects||Escherichia coli--United States--Prevention.|
|Additional Subjects||Escherichia coli ; Membrane filtration ; Water sampling ; Bacteria ; Agar ; Epidemiology ; Water pollution effects ; Marine environment ; Fresh water ; Health effects ; Gastrointestines ; Feces ; Substrates ; Surface waters ; Water quality ; Counting methods ; Calibration ; Contamination ; Laboratories ; Test methods|
The method describes a revised Escherichia coli membrane filter (MF) procedure, a single-step method that uses one medium, modified mTEC Agar, and does not require the transfer of the membrane filter to another medium or other substrate. The modified medium contains a chromogen (5-bromo-6-chloro- 3-indolyl--D-glucuronide), which is catabolized to glucuronic acid and a red- or magenta-colored compound byE. coli that produce the enzyme D-glucuronidase. The apparatus and equipment, and sampling, filtration, and verification procedures for this modified mTEC method are identical to those of the original mTEC method. Because the bacterium is a natural inhabitant only of the intestinal tract of warm-blooded animals, its presence in water samples is an indication of fecal pollution and the possible presence of entericpathogens.
"September 2002." "EPA-821-R-02-023." Includes bibliographical references. Microfiche.