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RECORD NUMBER: 26 OF 416

OLS Field Name OLS Field Data
Main Title Basis of the Insensitivity of 'Salmonella typhimurium' Strain TA98/1,8-DNP6 to the Mutagenic Action of Nitroarenes.
Author McCoy, E. C. ; Anders, M. ; Rosenkranz, H. S. ;
CORP Author Case Western Reserve Univ., Cleveland, OH.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1983
Report Number EPA-R-809231; EPA/600/J-83/336;
Stock Number PB86-188208
Additional Subjects Bacteria ; Toxicology ; Enzymes ; Reprints ; Salmonella typhimurium ; Nitroarenes ; Mutagenesis
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB86-188208 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 9p
Abstract
Salmonella typhimurium tester strain TA98/1,8-DNP6 is resistant to the mutagenicity of 1,8-dinitropyrene because it lacks an esterification enzyme which is needed for the formation of the ultimate mutagen, presumably the corresponding hydroxamic acid ester. This enzyme does not appear to be required for the activation of all nitroarenes and arylamines, as some of these are fully active in TA98/1,8-DNP. It is suggested that these form electrophilic arylnitrenium ions non-enzymatically from nitroso- and N-hydroxylamino-arenes intermediates. The esterification enzyme appears to be a transacetylase. An assay using 2-aminofluorene as the acetyl acceptor is described. Derivatives of S. typhimurium TA100 also lacking this enzyme were obtained by Tn5-mediated mutagenesis.