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RECORD NUMBER: 19 OF 415

OLS Field Name OLS Field Data
Main Title Assessment of Functional, Morphological and Enzymatic Test for Acute Nephrotoxicity Induced by Mercuric Chloride.
Author Lyle, G. M. ; Luthra, R. ; Bruckner, J. V. ; MacKenzie, W. F. ; Acosta, D. ;
CORP Author Texas Univ. Medical School at Houston.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1983
Report Number EPA-CR-807449; EPA-600/J-83-171;
Stock Number PB84-171008
Additional Subjects Mercuric chloride ; Toxicology ; Detection ; Assessments ; Enzymes ; Morphology ; Rats ; Laboratory animals ; Reprints ; Nephrotoxicity
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB84-171008 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/23/1988
Collation 21p
Abstract
The relative merits of a comprehensive series of contemporary methods for detection of acute nephrotoxicity were evaluated. Male Sprague-Dawley rats were given 0, 0.25, 0.5, 1.0, or 30.0 mg mercuric chloride (HgCl2)/kg body weight by ip injection. Indices of nephrotoxicity were examined 8, 24, 48, 72, and 96 h later. Alterations in urine osmolality, volume, and protein levels were seen within 24 h in response to 1 mg/kg or more of HgCl2. Administration of 0.5-3.0 mg/kg produced dose-dependent increases in urinary excretion of maltase activity and glucose by 24 h, the period of peak effect. There was no increase in maltase or alkaline phosphatase (AP) activity in the serum of these animals. Enzymuria was not apparent in rats that had marked elevations in serum AP, argininosuccinate lyase, and ornithine carbamyl transferase activities as a result of physical (i.e., dichlorodifluoromethane-frozen) or chemical (carbon tetrachloride-induced) damage of the liver.