||Bioluminescent Sensors for Detection of Bioavailable Hg(II) in the Environment.
Selifonova, O. ;
Burlage, R. ;
Barkay, T. ;
||University of West Florida, Pensacola. Center for Environmental Diagnostics and Bioremediation. ;Oak Ridge National Lab., TN. Environmental Sciences Div. ;Martin Marietta Energy Systems, Inc., Oak Ridge, TN.;Environmental Research Lab., Gulf Breeze, FL.;Department of Energy, Washington, DC. Office of Energy Research.
||EPA-R-818676-01-0, DE-AC05-84OR21400; EPA/600/J-94/008 ; CONTRIB-833
Water pollution monitoring ;
Mercury compounds ;
Aquatic ecosystems ;
Optical measuring instruments ;
Environmental chemistry ;
Fresh water ;
Vibrio fischeri ;
Escherichia coli ;
Inorganic mercury ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
Biosensors for the detection of pollutants in the environment can complement analytical methods by distinguishing bioavailable from inert unavailable forms of the contaminants. By using fusions of the well understood TN21 mercury resistance operon (mer) with promoterless luxCDABE from Vibrio fischeri, the authors have constructed and tested three biosensors to Hg(11). The utility of the biosensors in natural waters was demonstrated with freshwater, rain, and estuarine samples supplemented with HG(11). The biosensors described here semi-quantitatively detect bioavailable inorganic mercury in contaminated waters. (Copyright (c) 1993 American Society for Microbiology.)