Record Display for the EPA National Library Catalog

RECORD NUMBER: 4 OF 4

OLS Field Name OLS Field Data
Main Title Method A: Enterococci in Water by TaqMan (Trade Name) Quantitative Polymerase Chain Reaction (qPCR) Assay.
CORP Author Environmental Protection Agency, Washington, DC. Office of Water.
Year Published 2010
Report Number EPA/841/R-10/004
Stock Number PB2010-115385
Additional Subjects Bacteria ; Water pollution monitoring ; Fecal pollution ; Reagents ; Membrane filters ; Standards ; Equipment ; Supplies ; Safety ; Sampling ; Quality control ; Calibration ; Enteric pathogens ; Pollution prevention ; Waste management ; RNA gene sequences ; Quantitative polymerase chain reaction (qPCR) ; TaqMan ; Enterococci
Holdings
Library Call Number Additional Info Location Last
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Status
NTIS  PB2010-115385 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 01/21/2011
Collation 108p
Abstract
Method A describes a quantitative polymerase chain reaction (qPCR) procedure for the detection of DNA from enterococci bacteria in ambient water matrices based on the amplification and detection of a specific region of the large subunit ribosomal RNA gene (lsrRNA, 23S rRNA) from these organisms. The advantage of this method over currently accepted culture methods that require 24-48 hr to obtain results is its relative rapidity. Results can be obtained by this method in 3-4 hr, allowing same-day notifications of recreational water quality. While measurements of enterococci DNA by Method A and enterococci CFUs by culture methods such as EPA Method 1600 are performed for essentially the same purpose, i.e., to indicate fecal pollution, the results of these two approaches may not always be correlated with each other due to potential differences in the ratios of viable and non-viable bacteria in different water environments. Never-the-less, more recent epidemiological studies conducted at freshwater recreational beaches (Reference 17.3) have demonstrated similar or improved positive correlations between enterococci DNA measurements by this method and swimming-associated GI illness rates compared with those established for enterococci CFU measurements.