Record Display for the EPA National Library Catalog

RECORD NUMBER: 44 OF 73

OLS Field Name OLS Field Data
Main Title Plasmid Expression and Maintenance during Long-Term Starvation-Survival of Bacteria in Well Water.
Author Caldwell, B. A. ; Ye, C. ; Griffiths, R. P. ; Moyer, C. L. ; Morita, R. Y. ;
CORP Author Oregon State Univ., Corvallis.;Corvallis Environmental Research Lab., OR.
Publisher c1989
Year Published 1989
Report Number EPA-R-913413; EPA/600/J-89/243;
Stock Number PB90-137050
Additional Subjects Starvation ; Aquatic microbiology ; Water wells ; Bacteria ; Enterobacteriaceae ; Escherichia coli ; Pseudomonas ; Graphs(Charts) ; Reprints ; Plasmids ; Gene expression ; Gene products
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB90-137050 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/15/1990
Collation 7p
Abstract
Strains of enteric bacteria and pseudomonads containing plasmid R388::Tn1721(Tpr, Tcr) or pR0101 (Hgr, Tcr) were starved for over 250 days in sterile well water to evaluate effects of starvation-survival on plasmid expression and maintenance. Viable populations dropped to between approximately 0.1 and 1% of the initial populations. Escherichia coli(pR0101) and Pseudomonas cepacia (pR0101) lost both viability and plasmid expression at a lower rate than strains containing R388::Tn1721. Three patterns of host-plasmid interaction were detected: (i) no apparent loss of plasmid expression, (ii) loss of plasmid expression on initial recovery with subsequent expression upon resuscitation, and (iii) loss of capability to produce functional plasmid resistance. (Copyright (c) 1989, American Society for Microbiology.)