Main Title |
In vitro Study of Embryotoxic and Dysmorphogenic Effects of Mercuric Chloride and Methylmercury Chloride in the Rat. |
Author |
Kitchin, K. T. ;
Ebron, M. T. ;
Svendsgaard, D. ;
|
CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. |
Year Published |
1984 |
Report Number |
EPA-600/J-84-015; |
Stock Number |
PB84-197581 |
Additional Subjects |
Toxicology ;
Rats ;
Laboratory animals ;
In vitro analysis ;
Reprints ;
Mercuric chloride ;
Methylmercury chloride ;
Dysmorphogeny ;
Embryotoxicity
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB84-197581 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
9p |
Abstract |
Rat-embryo explants removed on day 10.5 of gestation were cultured for 48 hr in various concentrations of HgCl2 CH3HgCl or glutathione. Dose-related dysmorhogenesis and growth retardation occurred with increasing concentrations of HgCl2 (1-10 micro M). Increasing concentrations of CH3HgCl (3-100 micro M) produced a similar pattern of embryonic effects. The rat-serum incubation medium had no detectable level of reduced glutathione (0.02 mM) and only 0.12 mM total sulphydryl groups. Reduced glutathione (60-300 micro M) added to the incubation medium was relatively non-toxic. The addition of exogenous glutathione to culture medium containing HgCl2 partially antagonized the embryonic growth retardation and prevented most of the embryolethality observed in cultures to which only HgCl2 had been added. |