||Determination of Pentachlorophenol in Urine: The Importance of Hydrolysis.
Edgerton, Thomas R. ;
Moseman, R. F. ;
||Heath Effects Research Lab., Research Triangle Park, NC. Environmental Toxicology Div.
Chemical analysis ;
Biological extracts ;
Chlorine organic compounds ;
Electron capture gas chromatography ;
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A gas chromatographic method for more reliable determination of pentachlorophenol (PCP) in urine has been developed. After hydrolysis and extraction the sample was reacted with diazomethane to produce the methyl ether of PCP prior to analysis by electron-capture gas chromatography. An acid alumina column clean-up system was developed to remove interferences from the sample extracts and allow detectability of 1 ppb PCP. Average recoveries of greater than 90% were obtained from urine fortified with known amounts of PCP. The importance of hydrolysis and comparisons of present methodologies will also be presented.