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RECORD NUMBER: 616 OF 2796

OLS Field Name OLS Field Data
Main Title Differentiation of 'Giardia duodenalis' from Other 'Giardia spp.' by Using Polymerase Chain Reaction and Gene Probes.
Author Mahbubani, M. H. ; Bej, A. K. ; Perlin, M. H. ; Schaefer, F. W. ; Jakubowski, W. ;
CORP Author Louisville Univ., KY. Dept. of Biology.;Environmental Monitoring Systems Lab., Cincinnati, OH.
Publisher c1992
Year Published 1992
Report Number EPA-R-815955; EPA/600/J-92/038;
Stock Number PB92-150531
Additional Subjects Giardia ; Polymerase chain reaction ; DNA probes ; Aquatic microbiology ; Environmental monitoring ; Polyacrylamide gel electrophoresis ; Microbial sensitivity tests ; Deoxyribonucleic acids ; Taxonomy ; Reprints ; Giardia duodenalis ; Giardia muris ; Giardia ardeae
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NTIS  PB92-150531 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 08/28/1992
Collation 7p
Abstract
Giardia is a waterborne organism that is the most commonly identified pathogenic intestinal protozoan in the United States. Current detection techniques for Giardia in water include microscopy and immunofluorescence techniques. Speciation of the genus Giardia has been based on taxonomic criteria such as cell morphology and host specificity. Classification based on these criteria is inadequate for determining the different species of Giardia. The authors have developed a PCR-gene probe based detection system specific for Giardia, which can discriminate between the human relevant species of the G. duodenalis group and other Giardia species. The method can detect a single Giardia cyst and is therefore, sensitive enough for environmental monitoring.