||Plaquing and Immunological Methods for Detection and Identification of Enteroviruses.
Ludovic, Peter P. ;
Riggi, Rosella ;
Moor, Marion L. ;
||Arizona Univ., Tucson. Dept. of Microbiology and Medical Technology.
||OWRR-A-015-ARIZ; 01651,; A-015-ARIZ(1)
( Enteroviruses ;
( Immunology ;
Coxsackie viruses ;
ECHO viruses ;
Tissue culture ;
Waste water ;
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Various viral plaque assays were investigated using prototype viruses. In detailed studies using suspended agar and monolayer agar overlay procedures, neither primary nor secondary HAM cultures produced plaques with any of the viruses. HEp-2 plaqued poliovirus 1 but not echovirus 13 or Coxsackie B2. Wi38 plaqued poliovirus 1 and echovirus 13 but not Coxsackie B2. The monolayer agar overlay procedure was superior to the suspended cell agar method because fewer cells were required. When Wi38 was used to detect natural viruses, concentrates from sewage yielded no plaques in 5 samples whereas 4 of the 5 concentrates were virus-positive by CPE in fluid cultures. A plastic panel culture neutralization test was compared with the test tube method for the identification of viruses isolated from wastewater. Only one out of the eight viruses studied was different being identified as type 3 polio by the standard test and type 2 polio by the panel method. (Author)