||Formation and distribution of organic N-chloramines from the ingestion of chlorinated drinking water /
Scully, Frank E. ;
Sonenshine, D. E.
||Old Dominion Univ., Norfolk, VA.;Health Effects Research Lab., Research Triangle Park, NC.
|| U.S. Environmental Protection Agency, Health Effects Research Laboratory,
Chlorine--Physiological effect. ;
Drinking water--Health aspects. ;
Potable water ;
Amino acids ;
Chlorine organic compounds ;
Gastric juice ;
Chemical reactions ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||79 pages ; 28 cm
Because amino nitrogen compounds are important components of the average diet, the reactions of hypochlorite with amino compounds in the stomach were investigated. Stomach fluid was recovered from fasted Sprague-Dawley rats and the chlorine demand determined. An average volume-independent demand of 2.7 mg chlorine was measured. At doses below 40 mg/L chlorine reducing reactions appeared to account for reduction of all oxidizing species within 15 min as measured by the FAS-DPD titrimetric method. Part of the chlorine demand was associated with amino acids present in the stomach fluid. When stomach fluid was chlorinated to concentrations of chlorine between 200 and 1000 mg/L, organic N-chloramines were formed. Three chloramino acid derivatives, N-chloroalanine, N-chloroglycine, and N-chlorophenylalanine, were tentatively identified following derivatization with dansylsulfinic acid by cochromatography. The yield of organic chloroamines that could form in stomach fluid on administration of hypochlorite to animals was determined using tritiated piperidine as a model compound and doses of 200 and 1000 mg/L chlorine. A series of pharmacokinetic studies were conducted in male and female Sprague-Dawley rats employing (3)-N--chloropiperidine and (36)Cl-chloropiperidine as test compounds and (3)H- piperidine and (36)Cl-chloride as control compounds. A relationship between the retention of the (36)Cl activity in the pharmacokinetic studies and formation of (36)Cl-chloroorganic compounds in vitro is discussed.
Caption title. "September 1987." EPA/600/1-87/008. Microfiche.