||Chemical Separation and In situ Mutagenicity Testing.
Moeller, M. ;
Bjoerseth, A. ;
Houk, V. S. ;
||Health Effects Research Lab., Research Triangle Park, NC. ;Central Inst. for Industrial Research, Oslo (Norway).
Chemical analysis ;
Chromatographic analysis ;
Industrial wastes ;
Waste water ;
Paper industry ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
The traditional way to characterize a complex sample has been by chemical analysis to identify the individual components. However, unknown or overlooked mutagens or carcinogens may remain undetected by this method. In recent years a combination of chemical analysis and mutagenicity testing has been applied successfully to samples to provide a more complete assessment of mutagens in complex mixtures. The authors recently reported a new technique whereby complex samples were separated on thin-layer chromatography plates, and their mutagenic effect was registered directly on the developed plate by the Salmonella mutagenicity assay. This combined fractionation/bioassay scheme offers several advantages. Components of complex samples can be separated by one- or two- dimensional chromatography prior to exposure to the bacteria. By using the TLC plates, toxic compounds or components exhibiting synergistic/antagonistic effects may be separated from the mutagens and detected independently.