||Comparison of the Toxicity of Acrylamide, Cyclophosphamide, Chlrodecone, and Diethylstilbestrol in Chinese Hamster Ovary (CHO) Cells with Their Toxicity In vivo.
Simmons, J. E. ;
Jackson, M. ;
Lewtas, J. ;
Berman, E. ;
||Health Effects Research Lab., Research Triangle Park, NC. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
In vivo analysis ;
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In order to compare in vitro toxicity with in vivo toxicity, four chemicals that have been tested in the in vivo/in vitro toxicological screen proposed by the Health Effects Research Laboratory, EPA were tested in a Chinese Hamster Ovary (CHO) cytotoxicity assay. Viability index, cellular ATP concentration and rate of protein synthesis were decreased by exposure to acrylamide (AC), chlordecone (CH), diethylstilbestrol (DES), and cyclophosphamide (CY) (P<0.00001). All of these chemicals were active in the CHO cytotoxicity assay. The in vitro toxicity rankings (from most to least toxic) based either on cellular ATP concentration or rate of protein synthesis were identical: DES = CH < AC = CY. The toxicity ranking based on viability index was: DES = CH < AC < CY. All of these chemicals were toxic in vivo following 10 days of repeated dosing. The in vivo toxicity rankings (from most to least toxic) based either on mortality or on liver weight were identical: CY < AC < DES (unable to rank CH since highest concentration tested produced no change from control). The toxicity ranking for body weight at sacrifice was: CH = CY < AC (unable to rank DES since lowest concentration tested reduced body weight).