The objective of the paper is to provide a checklist of items to be addressed when compiling and reporting diatom and chrysophyte calibration data sets and accompanying ecological data and inference equations. The purpose of the list is to promote completeness and consistency in data together with proper presentation and adequate documentation, so that the data can be thoroughly evaluated and used fully and effectively by others. It is recommended that as many items on the list as possible and practical be addressed in all papers reporting calibration data sets submitted to scientific journals. The list is based on experience, reviewing papers on calibration data sets, discussions with calibration set developers and users, and attempts to compile data sets from several regions. The guidelines can be applied to calibration data sets based on other organisms as well.