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RECORD NUMBER: 9 OF 210

OLS Field Name OLS Field Data
Main Title Analytical Procedures and Quality Assurance Criteria for the Determination of Major and Minor Deoxynucleosides in Fish Tissue DNA by Liquid Chromatography-Ultraviolet Spectroscopy and Liquid Chromatography Thermospray Mass Spectrometry.
Author Serrano, J. ; Kuehl, D. W. ; Naumann, S. ;
CORP Author Environmental Research Lab.-Duluth, MN. ;AScI Corp., Duluth, MN.
Publisher c1993
Year Published 1993
Report Number EPA/600/J-93/277;
Stock Number PB93-222354
Additional Subjects Chemical analysis ; Deoxyribonucleic acids ; Nucleosides ; Fishes ; Liquid chromatography ; Ultraviolet spectrophotometry ; Mass spectroscopy ; Quality control ; Reprints ;
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB93-222354 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 11/22/1993
Collation 13p
Abstract
Analytical procedures and quality assurance criteria have been established for enzymatic hydrolysis of fish tissue DNA to free nucleosides and their subsequent characterization by liquid chromatography-photodiode-array ultraviolet spectroscopy and liquid chromatography-thermospray mass spectrometry. Optimization of enzymatic efficiency to assure minimal loss of modified nucleosides is described. Variability in analyte capacity factors and multiwavelength response have been compared for analyte standards and hydrolysates, and results have been used to derive qualitative and quantitative quality assurance criteria. A comparison of DNA mole percent calculated using single-wavelength quantification and multiwavelength averaging quantification indicates that less variability in data may be expected using the multiwavelength technique. Finally, the comparison of DNA from liver of three species of fish widely used in mutagen/carcinogen laboratory and field studies, rainbow trout (Onchorynchus mykiss), medaka (Oryzias latipes), and brown bullhead (Ictalurus nebulosus), has been determined. Identification of deoxyuridine in the DNA hydrolysates of each fish indicates that this analyte should be measured to accurately report DNA deoxynucleoside mole percent, especially when reporting data for the methylation of deoxycytidine. (Copyright (c) 1993 Elsevier Science Publishers B.V.)